All lanes : Anti-Granzyme A antibody [EPR20161] (ab209205) at 1/10000 dilution

Lane 1 : NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?


Exposure time: 1 second

This data was developed using ab209205, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The expression profile is consistent with what has been described in the literature (PMID: 15911377).

This data was developed using ab209205, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NK-92 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using ab209205, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human NK/T lymphoma tissue labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human NK/T lymphoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab209205, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labeling Granzyme A with ab209205 at 1/70 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

All lanes : Anti-Granzyme A antibody [EPR20161] (ab209205) at 1/1000 dilution

Lane 1 : Human spleen lysate at 10 µg
Lane 2 : Human tonsil lysate at 10 µg
Lane 3 : Human lymph node lysate at 10 µg
Lane 4 : Human lung lysate at 20 µg

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

This data was developed using ab209205, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 15 seconds, Lane 2: 3 minutes, Lane 3: 10 seconds, Lane 4: 5 seconds.

This data was developed using ab209205, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human spleen is observed [PMID: 9637500]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab209205, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Granzyme A with ab209205 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on some stromal cells of human endometrium is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.