Human Granzyme A ELISA Kit (ab255728)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.61 pg/ml
- Range: 31 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Granzyme A ELISA Kit
See all Granzyme A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 8 4.4% Inter-assay Sample n Mean SD CV% supernatant 3 5.6% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.61 pg/ml -
Range
31 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 93% - 107% Serum 88 87% - 90% Tissue Extracts 106 104% - 108% EDTA Plasma 88 83% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Granzyme A ELISA Kit (ab255728) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granzyme A protein in cell culture supernatant, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Granzyme A with 6.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Granzyme A, also known as Cytotoxic T-lymphocyte proteinase 1, is a member of the granzyme family of serine proteases encoded by the gene GZMA. Granzyme A is released by cytoplasmic granules in cytotoxic T cells and Natural killer cells, where it can induce caspase-independent cell death. Granzyme A is generally more abundant than the other four granzyme proteins found in humans. Granzyme A is expressed as a precursor with a 26- aa signal peptide, a 2- aa pro-peptide and a 234- aa mature chain.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Granzyme A Capture Antibody 1 x 600µl 10X Human Granzyme A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Granzyme A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
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Alternative names
- CTL tryptase
- CTLA3
- Cytolytic t cell and natural killer cell specific trypsin like serine protease
see all -
Database links
- Entrez Gene: 3001 Human
- Omim: 140050 Human
- SwissProt: P12544 Human
- Unigene: 90708 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Granzyme A standard curve in Sample Diluent NS.The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Granzyme A in human cell culture supernatant samples.The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC stimulated supernatant 50% and Jurkat cell supernatant 50%. Human PBMCs and Jurkat cells were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37ºC in the presence or absence of 1.5% PHAM. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 2,017 pg/mL in PBMC stimulated supernatant and 1,644 pg/mL in Jurkat cell supernatant.
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Interpolated concentrations of native Granzyme A in human lung extract based on a 500 µg/mL extract load.The concentrations of Granzyme A were measured in duplicate and interpolated from the Granzyme A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human Granzyme A ELISA Kit (ab255728)To learn more about the advantages of recombinant antibodies see here.
