Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (ab251095)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18716] to REDD-1/DDIT4 - BSA and Azide free
  • Suitable for: IP, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free
    See all REDD-1/DDIT4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18716] to REDD-1/DDIT4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa (untreated and treated with 0.5mM CoCL2 for 6 hours) cell lysate.

  • General notes

    Ab251095 is the carrier-free version of ab191871. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251095 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (ab251095)
    Western blot - Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (ab251095)
    All lanes : Anti-REDD-1/DDIT4 antibody [EPR18716] (ab191871) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : DDIT4 knockout HeLa cell lysate
    Lane 3 : HeLa treated with 0.5mM CoCL2 for 6 hours cell lysate
    Lane 4 : Untreated HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 25 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab191871).

    Lanes 1-4: Merged signal (red and green). Green - ab191871 observed at 35 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab191871 Anti-REDD-1/DDIT4 antibody [EPR18716] was shown to specifically react with REDD-1/DDIT4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264834 (knockout cell lysate ab257409) was used.  Wild-type and REDD-1/DDIT4 knockout samples were subjected to SDS-PAGE.  ab191871 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (ab251095)
    Anti-REDD-1/DDIT4 antibody [EPR18716] - BSA and Azide free (ab251095)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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