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Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (ab258340)

Price and availability

331 689 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Human CAMK2G (CamKII gamma) knockout HeLa cell lysate (ab258340)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human CAMK2G (CamKII gamma) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4 and 2 bp deletion in exon4.
  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261215 - Human CAMK2G knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Cellular localization

    Cell Membrane
  • Alternative names

    • CaM kinase II
    • CaM kinase II gamma chain
    • CaM kinase II subunit gamma
    • CAMK
    • CAMK II
    • CaMK II subunit gamma
    • CAMK2G
    • CAMKG
    • CAMKII
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261215 - Human CAMK2G knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Western blot - Human CAMK2G knockout HeLa cell lysate (ab258340)
    Western blot - Human CAMK2G knockout HeLa cell lysate (ab258340)

    Lane 1: Wild-type HeLa cell lysate (20 µg)

    Lane 2: CAMK2G knockout HeLa cell lysate (20 µg)

    Lane 3: T-47D cell lysate (20 µg)

    Lanes 1-3: Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.

    ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human CAMK2G knockout HeLa cell lysate (ab258340)
    Sanger Sequencing - Human CAMK2G knockout HeLa cell lysate (ab258340)

    Allele-1: 2 bp deletion in exon4

     

  • Sanger Sequencing - Human CAMK2G knockout HeLa cell lysate (ab258340)
    Sanger Sequencing - Human CAMK2G knockout HeLa cell lysate (ab258340)

    Allele-2: 1 bp deletion in exon4

     

  • Sanger Sequencing - Human CAMK2G knockout HeLa cell lysate (ab258340)
    Sanger Sequencing - Human CAMK2G knockout HeLa cell lysate (ab258340)

    Allele-3: 1 bp insertion in exon4

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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