Histamine Assay Kit (Fluorometric) (ab273277)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Food samples
Overview
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Product name
Histamine Assay Kit (Fluorometric)
See all Histamine kits -
Detection method
Fluorescent -
Sample type
Food samples -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Histamine Assay Kit (Fluorometric) (ab273277) can be used to identify low levels of histamine in various fish and products and sauces/beverages, as well as some biological samples. As little as 10 pmoles of histamine can be detected in samples, or roughly 10 parts per billion (ppb).
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Histamine Assay Buffer 1 x 25ml Histamine Developer (Lyophilized) 1 vial Histamine Enzyme Mix (Lyophilized) 1 vial Histamine Probe 1 x 0.2ml Histamine Standard (50 mM) 1 x 100µl Sample Clean-Up Mix (Lyophilized) 1 vial -
Research areas
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Relevance
Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. -
Cellular localization
Secreted -
Alternative names
- 2 (4 imidazolyl)ethylamine
- 4 (2 aminoethyl) 1 3 diazole
Images
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Histamine standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Estimation of Histamine concentration in soy sauce.Soy sauce was prepared according to above protocol. Histamine concentrations are: Regular Soy Sauce: 720 ± 100 μM, Low Sodium Soy Sauce: 1010 ± 20 μM.