Anti-C5 / C5b antibody [EPR23940-3] (ab275931)
![Anti-C5 / C5b antibody [EPR23940-3] (ab275931)](https://www.abcam.com/ps/products/275/ab275931/Images/ab275931-2-anti-c5c5b-antibody-epr239403-immunohistochemistry-human-breast.jpg "Anti-C5 / C5b antibody [EPR23940-3] (ab275931)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23940-3] to C5 / C5b
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-C5 / C5b antibody [EPR23940-3]
See all C5 / C5b primary antibodies -
Description
Rabbit monoclonal [EPR23940-3] to C5 / C5b -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma and liver tissue lysate; Mouse plasma; Rat plasma. IHC-P: Human liver and breast tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23940-3 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling C5/C5b with ab275931 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma of human breast. The section was incubated with ab275931 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)](https://www.abcam.com/ps/products/275/ab275931/Images/ab275931-412151-anti-c5c5b-antibody-epr239403-western-blot-ug1.jpg)
Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)All lanes : Anti-C5 / C5b antibody [EPR23940-3] (ab275931) at 1/1000 dilution
Lane 1 : Mouse plasma
Lane 2 : Rat plasma
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 188 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 23401592, 25387724).
Exposure time: 48 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)](https://www.abcam.com/ps/products/275/ab275931/Images/ab275931-1-anti-c5c5b-antibody-epr239403-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling C5/C5b with ab275931 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver. The section was incubated with ab275931 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)](https://www.abcam.com/ps/products/275/ab275931/Images/ab275931-412150-anti-c5c5b-antibody-epr239403-western-blot-ug.jpg)
Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)All lanes : Anti-C5 / C5b antibody [EPR23940-3] (ab275931) at 1/1000 dilution
Lane 1 : Human plasma at 10 µg
Lane 2 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 188 kDa
Observed band size: 190,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
The molecular weight observed is consistent with what has been described in the literature (PMID: 24071736, 23401592, 25387724)
Exposure time: 59 seconds.
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![Anti-C5 / C5b antibody [EPR23940-3] (ab275931)](https://www.abcam.com/ps/products/275/ab275931/Images/ab275931-5-benefits-of-recombinant-antibodies.png)
Anti-C5 / C5b antibody [EPR23940-3] (ab275931)