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Cell Biology Other Antibodies

Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)

Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12282-87] to LSM12 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free
    See all LSM12 primary antibodies
  • Description

    Rabbit monoclonal [EPR12282-87] to LSM12 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human LSM12. The exact sequence is proprietary.
    Database link: Q3MHD2

  • General notes

    ab249753 is the carrier-free version of ab173292.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12282-87
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    Western blot - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    All lanes : Anti-LSM12 antibody [EPR12282-87] (ab173292) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa



    This data was developed using ab173292, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    This data was developed using ab173292, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LSM12 with ab173292 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    Immunocytochemistry - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    This data was developed using ab173292, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173292 at 1/100 dilution.
  • Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    This data was developed using ab173292, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from 1) HeLa cell lysate or 2) 1X PBS using ab173292 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)
    Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (ab249753)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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