Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free
    See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250743 is the carrier-free version of ab184302. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250743 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

    Lane 1 : Mouse cerebellum tissue lysate
    Lane 2 : Rat cerebellum tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa
    Observed band size: ~300 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab184302, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID: 8463825, 22528491), though results may vary depending on percentage acrylamide gel used.

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution + Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa


    Exposure time: 3 minutes


    This data was developed using ab184302, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID: 8463825; 22528491), though results may vary depending on percentage acrylamide gel used.

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 102 kDa


    Exposure time: 3 minutes


    This data was developed using ab184302, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID: 8463825, 22528491), though results may vary depending on percentage acrylamide gel used.

  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    This data was developed using ab184302, the same antibody clone in a different buffer formulation.Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse cerebellum lysate, 10μg (Input). Lane 2: ab184302 IP in Mouse cerebellum lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    This data was developed using ab184302, the same antibody clone in a different buffer formulation.Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Rat cerebellum lysate, 10μg (Input). Lane 2: ab184302 IP in Rat cerebellum lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)

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