Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Parkin / PARK

Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)

Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4117] to PGP9.5 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Human PARK7 Antibody Pair - BSA and Azide free (DJ1) (ab241845)
Product image
Anti-AIMP2/p38 antibody (ab228004)
Product image
Human UCHL1 Antibody Pair - BSA and Azide free (ab244190)
Anti-PARK7/DJ1 antibody - BSA and Azide free (Capture) (ab278011)

Overview

  • Product name

    Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free
    See all PGP9.5 primary antibodies
  • Description

    Rabbit monoclonal [EPR4117] to PGP9.5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGP9.5 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
  • General notes

    Ab176838 is the carrier-free version of ab109261. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab176838 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 7.20 x 10 -10 M
    Learn more about KD
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4117
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Parkin / PARK
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)

    This IHC data was generated using the same anti-PGP9.5 antibody clone, EPR4117, in a different buffer formulation (cat# ab109261).

    ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)
    Western blot - Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)

    This WB data was generated using the same anti-PGP9.5 antibody clone, EPR4117, in a different buffer formulation (cat# ab109261).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
    Lane 2: UCHL1 (KO) knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)              

    Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)
    Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free (ab176838)

  •  
  • Product image

    Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)

    Applications: ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB

  •  
  • Product image

    Anti-PGP9.5 antibody [UCHL1/775] - BSA and Azide free (ab212934)

    Applications: IHC-P

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

  •  
  • Product image

    Anti-PAK1+PAK2+PAK3 (phospho S141 + S144 + S154) antibody [EP656Y] - BSA and Azide free (ab239830)

  •  
  • Product image

    Anti-MLKL antibody [EPR17514] - BSA and Azide free (ab211045)

  •  
  • Product image

    HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)

  •  
  • Product image

    Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)

  •  
  • Product image

    Anti-CD62L antibody - C-terminal (ab135792)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.