Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)

Key features and details

  • Mouse monoclonal [13C4 / I3C4] to PGP9.5 - BSA and Azide free
  • Suitable for: WB, IHC-FoFr, IHC-P, IHC-Fr, ICC, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free
    See all PGP9.5 primary antibodies

  • Description

    Mouse monoclonal [13C4 / I3C4] to PGP9.5 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-FoFr, IHC-P, IHC-Fr, ICC, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Rabbit, Guinea pig, Dog, Pig

  • Immunogen

    corresponding to PGP9.5.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. Human, mouse and rat brain tissue lysate. Rat cortex tissue lysate. SHSY-5Y whole cell lysate. Human spinal cord tissue lysate. IHC-P: Rat pancreas tissue.

  • General notes

    ab180872 is the PBS only version of ab8189.

    This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)
    Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)

    Image produced using the same antibody clone but different formulation, ab8189.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: PGP9.5 knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab8189 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab8189 was shown to specifically react with PGP9.5 in wild-type HAP1 cells as signal was lost in PGP9.5 knockout cells. Wild-type and PGP9.5 knockout samples were subjected to SDS-PAGE. ab8189 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)

    Image produced using the same antibody clone but different formulation, ab8189.

    IHC image of PGP9.5 staining in rat pancreas formalin-fixed, paraffin-embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8189, 0.02µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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