Aldose Reductase Activity Kit (Colorimetric) (ab273276)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 100 µU
Overview
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Product name
Aldose Reductase Activity Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
100 µU -
Assay duration
Multiple steps standard assay -
Product overview
Aldose Reductase Activity Assay Kit (Colorimetric) (ab273276) utilizes the ability of Aldose Reductase (AR) to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. The assay has been optimized to be monitored using a 96 well plate. The kit can detect as low as 0.1 mU. The assay kit is simple and can be used in a high-throughput format.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Aldose Reductase (Lyophilized) 1 vial AR Assay Buffer 1 x 35ml AR Substrate 1 x 1ml DTT (1 M) 1 x 0.4ml NADPH (Lyophilized) 1 vial UV transparent plate (96-well) 1 unit -
Research areas
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Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. -
Tissue specificity
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- ADR
- AKR1B 1
- Akr1b1
see all
Images
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NADPH standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Purified Aldose Reductase activityPurified Aldose Reductase activity.
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Aldose Reductase Activity in Tissue SamplesAldose Reductase specific activity was calculated in Rat Kidney tissue lysates (20 µg protein) and HepG2 cell lysates (110 µg protein).