Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-FKBP52 antibody [EPR21120] (ab230952)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21120] to FKBP52
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-FKBP52 antibody [EPR21120]
See all FKBP52 primary antibodies
Description

Rabbit monoclonal [EPR21120] to FKBP52
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, MCF7, HeLa, HEK-293T and RAW 264.7 whole cell lysate. Mouse and rat brain lysate. Human fetal kidney lysate. IHC-P: Human breast cancer tissue. Mouse and rat cerebrum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21120
Isotype

IgG
Research areas

Western blot - Anti-FKBP52 antibody [EPR21120] (ab230952)

Lanes 1-3 & 7 : Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/5000 dilution
Lanes 4-6 & 8 : Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/1000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 5 : Mouse brain lysate at 10 µg
Lane 6 : Rat brain lysate at 10 µg
Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 8 : Human fetal kidney lysate at 10 µg

Secondary
Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 8 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-3: 81 seconds; Lane 4: 30 second; Lanes 5: 120 seconds; Lane 6-8: 3 minutes.

The molecular mass observed is consistent with what has been described in the literature (PMID: 26065228).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR21120] (ab230952)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling FKBP52 with ab230952 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in human breast cancer (PMID: 19584157) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR21120] (ab230952)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling FKBP52 with ab230952 at 1/1,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in rat cerebrum (PMID: 20133804; PMID: 20422297) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR21120] (ab230952)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling FKBP52 with ab230952 at 1/1,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in mouse cerebrum (PMID: 20133804; PMID: 20422297) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-FKBP52 antibody [EPR21120] (ab230952)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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