Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FKBP52 antibody [EPR6618] (ab129097)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6618] to FKBP52
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-FKBP52 antibody [EPR6618]
    See all FKBP52 primary antibodies

  • Description

    Rabbit monoclonal [EPR6618] to FKBP52

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)
    Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)
    All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : K562 cell lysates
    Lane 4 : MCF7 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)

    ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)

    ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)

    ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097)
    OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FKBP52 antibody [EPR6618] (ab129097)
    Anti-FKBP52 antibody [EPR6618] (ab129097)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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