Anti-EIF3D antibody (ab264228)
Key features and details
- Rabbit polyclonal to EIF3D
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EIF3D antibody
See all EIF3D primary antibodies -
Description
Rabbit polyclonal to EIF3D -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
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Immunogen
Synthetic peptide within Human EIF3D aa 150-200. The exact sequence is proprietary.
Database link: O15371 -
Positive control
- WB: HEK-293T whole cell lysate; IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EIF3D antibody (ab264228)All lanes : Anti-EIF3D antibody (ab264228) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293-T whole cell lysate at 50 µgDetected with chemiluminescence with exposure time of 3 minutes.
4-20% SDS-PAGE
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Immunoprecipitation - Anti-EIF3D antibody (ab264228)EIF3D was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264228 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264228 at 0.4 µg/ml.
Lane 1: ab264228 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
