Human Fetuin-A ELISA Kit (ab269372)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 116.24 pg/ml
- Range: 203.13 pg/ml - 13000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fetuin-A ELISA Kit
See all AHSG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.3% Inter-assay Sample n Mean SD CV% Serum 3 5.6% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
116.24 pg/ml -
Range
203.13 pg/ml - 13000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 117 104% - 124% Serum 112 104% - 125% EDTA Plasma 115 108% - 119% Cit plasma 110 102% - 125% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fetuin-A ELISA Kit (ab269372) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fetuin-A protein in cell culture supernatant, edta plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fetuin-A with 116.24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Fetuin A is a highly abundant protein secreted by the liver that is capable of binding a wide variety of ligands, similar to serum albumin. A member of the cystatin family, Fetuin A plays a vital role in preventing tissue calcification and is also involved in fatty acid transport, insulin regulation and anti-inflammatory responses. Fetuin A is encoded by a single gene on chromosome 3, contains two cystatin domains, and exists as a heterodimer in its posttranslational form. Fetuin A is more abundant than serum albumin in fetal serum.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fetuin-A Capture Antibody 1 x 600µl 10X Human Fetuin-A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Fetuin-A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. -
Tissue specificity
Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. -
Sequence similarities
Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 59 kDa bone sialic acid-containing protein
- A2HS
- Aa2-066
see all -
Database links
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
Images
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SimpleStep ELISA Protocol Diagram
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Human Fetuin-A standard curve comparisonStandard Curve comparison between human Fetuin-A SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human MMP3 standard curve comparisonStandard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab185989) and current human MMP3 SimpleStep ELISA kit (ab269372).
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Example of human Fetuin-A standard curve in Sample Diluent 25BS.The Fetuin-A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Fetuin-A in human serum, plasma and cell culture supernatant samples.The concentrations of Fetuin-A were measured in duplicates, interpolated from the Fetuin-A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 20,000, plasma (citrate) 1: 20,000, plasma (EDTA) 1: 20,000, and HepG2 supernatant 1: 600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin-A concentration was determined to be 194.15 µg/mL in serum, 164.51 µg/mL in plasma (citrate), 172.44 µg/ml in plasma (EDTA), and 3.85 µg/mL HepG2 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin-A concentration was determined to be 170.03 µg/mL with a range of 136.89 – 246.74 µg/mL.
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Sandwich ELISA - Human Fetuin-A ELISA Kit (ab269372)To learn more about the advantages of recombinant antibodies see here.
