Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CLIC1 antibody [EPR22907-50] (ab229917)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22907-50] to CLIC1
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-CLIC1 antibody [EPR22907-50]
See all CLIC1 primary antibodies
Description

Rabbit monoclonal [EPR22907-50] to CLIC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC/IF	Mouse Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal heart, kidney and spleen lysates, K-562, C2C12, U-87 MG and MDA-MB-231 whole cell lysates, Mouse kidney and spleen lysates, Rat spleen lysate, C6 and RAW 264.7 whole cell lysates; ICC/IF: C2C12 and K-562 cells; Flow Cyt: K-562 and C2C12; IP: Mouse placenta lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22907-50
Isotype

IgG
Research areas

Western blot - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

All lanes : Anti-CLIC1 antibody [EPR22907-50] (ab229917) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat spleen lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 5 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure times.
Lanes 1-2: 15 seconds;
Lane 3: 3 minutes;
Lanes 4-5: 48 seconds.

Blocking buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

All lanes : Anti-CLIC1 antibody [EPR22907-50] (ab229917) at 1/1000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 4 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure times.
Lane 1:26 seconds;
Lane 2:10 seconds;
Lanes 3-4: 3 minutes.
Western blot - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

All lanes : Anti-CLIC1 antibody [EPR22907-50] (ab229917) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

CLIC1 was immunoprecipitated from 0.35mg Mouse placenta lysate with ab229917 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab229917 at 1/1000 dilution (0.5 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1: Mouse placenta lysate 10μg

Lane 2: ab229917 IP in Mouse placenta lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229917 in Mouse placenta lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cells labelling CLIC1 with at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in K-562 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labelling CLIC1 with at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in C2C12 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Flow Cytometry - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling CLIC1 with ab229917 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-CLIC1 antibody [EPR22907-50] (ab229917)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling CLIC1 with ab229917 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-CLIC1 antibody [EPR22907-50] (ab229917)

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