Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16621-67] to Hsp90 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free
    See all Hsp90 primary antibodies

  • Description

    Rabbit monoclonal [EPR16621-67] to Hsp90 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Rat
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240366 is the carrier-free version of ab203126. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Immunocytochemistry/Immunofluorescence analysis of 100% methanol-fixed NIH/3T3 (Mouse embryonic fibroblast) cells labeling Hsp90 alpha + beta with ab203126 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Control - PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Immunocytochemistry/Immunofluorescence analysis of 100% methanol-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Hsp90 alpha + beta with ab203126 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Control - PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Hsp90 alpha + beta with ab203126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and weak nucleus staining on germ cells of Human testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Hsp90 alpha + beta with ab203126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and weak nucleus staining on tumor cells of Human lung cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Hsp90 alpha + beta with ab203126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and nucleus staining on neuron of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Hsp90 alpha + beta with ab203126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm and weak nucleus staining on germ cells of rat testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Flow Cytometry - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Hsp90 alpha + beta with ab203126 at 1/350 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

  • Immunoprecipitation - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Immunoprecipitation - Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

    Hsp90 alpha + beta was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab203126 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab203126 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).

    Lane 2: ab203126 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203126 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203126).

  • Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)
    Anti-Hsp90 antibody [EPR16621-67] - BSA and Azide free (ab240366)

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