Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12700] to Human IgG - BSA and Azide free
  • Suitable for: ELISA, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Human IgG antibody [EPR12700] - BSA and Azide free
    See all Human IgG primary antibodies

  • Description

    Rabbit monoclonal [EPR12700] to Human IgG - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Human IgG. Isolated from a pool of normal Human sera.

  • General notes

    Ab250451 is the carrier-free version of ab181236. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250451 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12700

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using ab181236, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (purified) + Human tonsil lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 36, 41 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    This data was developed using ab181236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (purified) + Human spleen tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 36, 41 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    This data was developed using ab181236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (unpurified)

    Lane 1 : Human plasma lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human tonsil lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 36, 41 kDa



    This data was developed using ab181236, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    This data was developed using ab181236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (unpurified)

    Lane 1 : Human IgG
    Lane 2 : Human IgM
    Lane 3 : Human IgA
    Lane 4 : Human IgD

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36, 41 kDa



    This data was developed using ab181236, the same antibody clone in a different buffer formulation.

     

  • Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)
    Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

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