HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)
![HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)](https://www.abcam.com/ps/products/197/ab197740/Images/ab197740-248592-anti-tubulin-antibody-yl1-2-loading-control-hrp-immunohistochemistry.jpg "HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)")
Key features and details
- HRP Rat monoclonal [YL1/2] to Tubulin - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-Tubulin antibody [YL1/2] - Loading Control
See all Tubulin primary antibodies -
Description
HRP Rat monoclonal [YL1/2] to Tubulin - Loading Control -
Host species
Rat -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, a wide range of other species, Mammals, African green monkey
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Immunogen
Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.
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Epitope
A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). -
Positive control
- WB: HeLa, NIH 3T3 and PC12 whole cell lysates. IHC-P: Normal human colon tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
YL1/2 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)
IHC image of Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197740, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)](https://www.abcam.com/ps/products/197/ab197740/Images/ab197740-246649-anti-tubulin-antibody-yl1-2-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)All lanes : HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197740 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
