Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Kallikrein 10 ELISA Kit (ab279406)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5.29 pg/ml
  • Range: 10.94 pg/ml - 700 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Kallikrein 10 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.6%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    5.29 pg/ml

  • Range

    10.94 pg/ml - 700 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 113 110% - 115%
    Serum 101 91% - 110%
    Hep Plasma 101 92% - 107%
    EDTA Plasma 103 86% - 112%
    Cit plasma 107 102% - 115%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Kallikrein 10 ELISA kit (ab279406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Kallikrein 10 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kallikrein 10 with 5.29 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Kallikrein 10 is a member of a family of serine proteases that are involved in a variety of cellular functions. Kallikrein 10 has been linked to carcinogenesis as having a tumor suppressor role in breast and prostate cancer. Kallikrein 10 is upregulated in gastrointestinal cancers but downregulated in breast cancer, prostate cancer, NSC lung cancer and renal call carcinoma.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Recombinant Antibody benefits
    Recombinant Antibody benefits

    To learn more about the advantages of recombinant antibodies see here.

  • Example of human Kallikrein 10 standard curve in Sample Diluent NS.
    Example of human Kallikrein 10 standard curve in Sample Diluent NS.

    The Kallikrein 10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Kallikrein 10 in human serum and plasma samples.
    Interpolated concentrations of native Kallikrein 10 in human serum and plasma samples.

    The concentrations of Kallikrein 10 were measured in duplicates, interpolated from the Kallikrein 10 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 10 concentration was determined to be 263.09 pg/mL in serum, 526.64 pg/mL in plasma (citrate), 380.88 pg/mL in plasma (heparin), and 195.75 pg/mL in plasma (EDTA).

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 10 concentration was determined to be 1,062.93 pg/mL with a range of 728.05 – 1,332.52 pg/mL.

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