Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12927] to Caspase-14 - BSA and Azide free
Suitable for: IHC-P, ICC/IF, WB, IP, Flow Cyt
Reacts with: Rat, Human

Product name

Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free
See all Caspase-14 primary antibodies
Description

Rabbit monoclonal [EPR12927] to Caspase-14 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab249835 is the carrier-free version of ab174847. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12927
Isotype

IgG
Research areas

Western blot - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

All lanes : Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : ECV-304 cell lysate
Lane 3 : HACAT cell lysate
Lane 4 : Human skin lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 28 kDa

This data was developed using ab174847, the same antibody clone in a different buffer formulation.
Immunocytochemistry - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.
Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1:200.-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using ab174847 at a 1:10 dilution (red), or rabbit IgG negative control (green).
Immunoprecipitation - Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

This data was developed using ab174847, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-Caspase-14 antibody [EPR12927] - BSA and Azide free (ab249835)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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