Anti-Bim (phospho S65) antibody (ab17935)
Key features and details
- Rabbit polyclonal to Bim (phospho S65)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Bim (phospho S65) antibody
See all Bim primary antibodies -
Description
Rabbit polyclonal to Bim (phospho S65) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
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Positive control
- Hek293T co-expressing rat Bim and active MEKK1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bim (phospho S65) antibody (ab17935)
Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2, 5) or with
mutant S65A Bim and MEKK1 (3, 6), were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or ab17935 (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with a goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.
Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2
The data show that the signal is detected only in lysates co-expressing rat WT Bim and active MEKK1. This signal is abolished in cells expressing mutant rat Bim S65A and MEKK1, verifying that the signal is site-phosphospecific.
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