Human PlGF ELISA Kit (ab260056)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.4 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PlGF ELISA Kit
See all PLGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.9% Inter-assay Sample n Mean SD CV% Serum 3 2% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.4 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 113 112% - 114% Urine 86 83% - 91% Serum 86 85% - 88% Cell culture media 98 93% - 101% Hep Plasma 102 98% - 105% EDTA Plasma 99 96% - 103% Cit plasma 93 92% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PlGF ELISA Kit (ab260056) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PlGF protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PlGF with 4.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PIGF (placenta growth factor) is a secreted growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. Four PIGF isoforms are produced by alternative splicing, three of them are present in placental tissue. Only PlGF-1 is found in the colon and mammary carcinomas. PIGF-3 binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PlGF Capture Antibody 1 x 600µl 10X Human PlGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human PlGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
Tissue specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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Alternative names
- D12S1900
- Pgf
- PGFL
see all -
Database links
- Entrez Gene: 5228 Human
- Omim: 601121 Human
- SwissProt: P49763 Human
- Unigene: 252820 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PIGF standard curve in Sample Diluent NS.The PIGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PIGF in human pregnant serum and JAR cell culture supernatant samples.The concentrations of PIGF were measured in duplicates, interpolated from the PIGF standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 50% and JAR cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PIGF concentration was determined to be 1,147 pg/mL in neat pregnant serum and 11,000 pg/mL in neat JAR cell culture supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PIGF concentration was determined to be 38.42 pg/mL in detectable samples with a range of ND – 44.05 pg/mL for all samples.
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Sandwich ELISA - Human PlGF ELISA Kit (ab260056)To learn more about the advantages of recombinant antibodies see here.
