Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody (ab59196)
Key features and details
- Rabbit polyclonal to JNK1+JNK2+JNK3 (phospho T183 + Y185)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody
See all JNK1+JNK2+JNK3 primary antibodies -
Description
Rabbit polyclonal to JNK1+JNK2+JNK3 (phospho T183 + Y185) -
Host species
Rabbit -
Specificity
ab59196 detects endogenous levels of JNK1/2/3 only when phosphorylated at threonine183/tyrosine 185. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 183/tyrosine 185 (MMTPPYPVV)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody (ab59196)All lanes : Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody (ab59196) at 1/500 dilution
Lane 1 : 293 cell extract treated with UV (5 mins)
Lane 2 : 293 cell extract treated with UV (5 mins) with immunising phosphopeptide
Predicted band size: 48 kDa
Observed band size: 40 and 60 kDa why is the actual band size different from the predicted?
