Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16797-211] to JNK1+JNK2+JNK3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis, Recombinant fragment

Overview

  • Product name

    Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] - BSA and Azide free
    See all JNK1 + JNK2 + JNK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16797-211] to JNK1+JNK2+JNK3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis, Recombinant fragment
    Predicted to work with: Monkey

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human JNK1, JNK2 and JNK3 full length recombinant proteins; K562, HeLa, Jurkat, Neuro-2a, UMNSAH/DF-1, MDCK, MDBK and COS-1 whole cell lysates; Zebrafish and X. tropicalis lysates. Mouse brain, Rat brain, Rat heart, RAW 264.7, PC-12 and NIH/3T3 lysates. ICC/IF: HeLa cells. IP: Jurkat whole cell extract. Flow Cyt (intra): HeLa cells

  • General notes

    ab225572 is the carrier-free version of ab179461.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
    Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with purified ab179461 at 1/180 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179461).

  • Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
    Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)

    JNK1+JNK2+JNK3 were immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract with ab179461 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179461 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Jurkat whole cell extract. Lane 2: PBS instead of Jurkat whole cell extract.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179461).

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
    Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)

    This ICC/IF data was generated using the same anti-JNK1/2/3 antibody clone [EPR16797-211] in a different buffer formulation (cat# ab179461).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with ab179461 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: - ab179461 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
    Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)

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