Anti-ERK8 (phospho T175 + Y177) antibody (ab73209)
Key features and details
- Rabbit polyclonal to ERK8 (phospho T175 + Y177)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK8 (phospho T175 + Y177) antibody
See all ERK8 primary antibodies -
Description
Rabbit polyclonal to ERK8 (phospho T175 + Y177) -
Host species
Rabbit -
Specificity
ab73209 detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177 (Human) or threonine 176 and tyrosine 178 (Mouse).
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human ERK8 (phospho T175 + Y177).
Sequence:AVTPEYPVA
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Positive control
- Jurkat cell extract.
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General notes
Previously labelled as MAPK15.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab73209 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERK8 (phospho T175 + Y177) antibody (ab73209)All lanes : Anti-ERK8 (phospho T175 + Y177) antibody (ab73209) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
