Anti-MAP3K4 antibody (ab186125)
Key features and details
- Rabbit polyclonal to MAP3K4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MAP3K4 antibody
See all MAP3K4 primary antibodies -
Description
Rabbit polyclonal to MAP3K4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human MAP3K4 aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_005913.2).
Database link: Q9Y6R4 -
Positive control
- 293T and Jurkat whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab186125 was affinity purified using an epitope specific to MAP3K4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MAP3K4 antibody (ab186125)All lanes : Anti-MAP3K4 antibody (ab186125) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 182 kDa
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-MAP3K4 antibody (ab186125)Detection of MAP3K4 in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186125 at 6 µg/reaction for IP (Lane 1). For WB detection, ab186125 was used at 1µg/ml. Lane 2: Control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
