Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] - BSA and Azide free (ab239886)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1597Y] to JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] - BSA and Azide free
See all JNK1+JNK2+JNK3 primary antibodies -
Description
Rabbit monoclonal [EP1597Y] to JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa cell lysate; WB: Lysate from UV-treated HeLa, HEK-293, NIH/3T3, and C6 cells.
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General notes
Ab239886 is the carrier-free version of ab76572. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239886 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1597Y -
Isotype
IgG -
Research areas
Images
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Lanes 1 & 3-6 : Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] (ab76572) at 1/5000 dilution (Purified)
Lane 2 : Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] (ab76572) at 5000 cells
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 200J/m2 UV-C then recovery for 30 minutes whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 20 J/m2 UV-C then recovery for 1 hour whole cell lysates
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 6 : C6 (Rat glial tumor glial cell) treated with 40 J/m2 UV-C then recovery for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76572).
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Purified ab76572 at 1/70 dilution (2ug) immunoprecipitating JNK1+JNK2+JNK3 in HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate (10µg)
Lane 2 (+): ab76572 + HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76572 in HeLa treated with 20J/m2 UV-C then recovery for 1h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 46, 54 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76572). -
Lane 1: Human JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) phospho peptide
Lane 2: Human JNK1+JNK2+JNK3 non-phospho pepetideStained with ab76572 at a 1:1000 dilution. Dection was with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1:100,000 dilution.
Exposure: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76572).
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