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Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18841-95] to JNK1+JNK2+JNK3 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free
    See all JNK1+JNK2+JNK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18841-95] to JNK1+JNK2+JNK3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human JNK1, JNK2 and JNK3 full length recombinant proteins; Human fetal liver, fetal heart and fetal kidney lysates; Jurkat, HeLa, K562, MCF7, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, kidney and spleen lysates. ICC/IF: HeLa and NIH/3T3 cell lines. Flow Cyt: Jurkat and HeLa cell lines. IP: HeLa whole cell lysate.

  • General notes

    Ab218200 is the carrier-free version of ab208035. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab218200 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)
    Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm and weak nuclear staining on HeLa cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab208035 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)
    Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm staining on NIH/3T3 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab208035 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)
    Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)
    Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)
    Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

    JNK1+JNK2+JNK3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab208035 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab208035 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10ug (Input).

    Lane 2: ab208035 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208035 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)
    Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

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