Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22116] to Histone H4 (unmodified K20) - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free
    See all Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22116] to Histone H4 (unmodified K20) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Peptide array data showed that this RabMAb bound three methylated peptides (R19me1, R19me2a and R23me1) but with lower affinity than the unmethylated peptide.

  • Tested applications

    Suitable for: IHC-P, WB, IP, ChIPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue.

  • General notes

    Ab236241 is the carrier-free version of ab227804. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236241 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22116

  • Isotype

    IgG

Images

  • ChIP - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    ChIP - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab227804 (red), and 20 µl of protein A/G sepharose beads slurry (10 µl of sepharose A beads + 10 µl of sepharose G beads). 2 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227804).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H4K20 with ab227804 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sporadic nuclear staining in human colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227804).

  • Immunoprecipitation - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    Immunoprecipitation - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

    Histone H4 (unmodified K20) was immunoprecipitated from 0.35 mg of SET8 depleted U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with ab227804 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227804 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: SET8 depleted U-2 OS whole cell lysate 10 μg (Input).

    Lane 2: ab227804 IP in SET8 depleted U-2 OS whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227804 in SET8 depleted U-2 OS whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Depletion of Set8, a histone methyltransferase, delays S-phase progression and impairs DNA replication (PMID: 18166648). It also prevents the monomethylation of H4K20, which is normally mediated by Set8 (PMID: 18166648).

    This lysate was kindly provided by our collaborator Dr. Anja Groth, Biotech Research and Innovation Centre (BRIC). University of Copenhagen.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227804).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Histone H4K20 with ab227804 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sporadic nuclear staining in human bladder cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227804).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Histone H4K20 with ab227804 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in mouse spleen is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227804).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Histone H4K20 with ab227804 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sporadic nuclear staining in rat spleen is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227804).

  • Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)
    Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Histone H4 (unmodified K20) antibody [EPR22116] - BSA and Azide free (ab236241)

© 2021 Astana Biomed Group LLP. All rights reserved.