All lanes : Anti-p53 antibody (ab131442) at 1/500 dilution

Lane 1 : Wild type HAP1 whole cell lysate (20 µg)
Lane 2 : p53 knockout HAP1 whole cell lysate (20 µg)
Lane 3 : Saos2 (20 µg)
Lane 4 : A431 (20 µg)

Performed under reducing conditions.

Predicted band size: 53 kDa

 Lanes 1 - 4: Merged signal (red and green). Green - ab131442 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

Wild-type and p53 knockout samples were subjected to SDS-PAGE. ab131442 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Other recommended antibodies to this target are ab32389 and ab26.

ChiP analysis of ab131442. Expression of p21

Immunohistochemical analysis of rat colon tissue sections with ab131442 at 1/150 dilution.The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4oC. A goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling p53with ab131442 at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (RT, 30min).
Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling p53with ab131442 at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (RT, 30min).
Negative control was used by secondary antibody only.

All lanes : Anti-p53 antibody (ab131442) at 1/1000 dilution

Lane 1 : Rat breast tissue lysate
Lane 2 : Rat colon tissue lysate

Lysates/proteins at 40 µg per lane.

Predicted band size: 53 kDa

ab131442 staining p53 in formalin -fixed, paraffin embedded Rat C6 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1/100 dilution. A goat anti-rabbit IgG H&L (1/50 dilution) was used as the secondary antibody. 

 

Immunohistochemical analysis of rat breast tissue sections with ab131442 at 1/150 dilution. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4oC. A goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat breast tissue with ab131442 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary. 

All lanes : Anti-p53 antibody (ab131442) at 1/600 dilution

Lane 1 : Mouse Brain
Lane 2 : Mouse Lung
Lane 3 : HL60 Cells

Predicted band size: 53 kDa


Exposure time: 5 seconds

ChiP analysis of ab131442 in 293T cells at a dilution of 1:50.

All lanes : Anti-p53 antibody (ab131442) at 1/500 dilution

Lane 1 : MDA cell extract
Lane 2 : JK cell extract

Predicted band size: 53 kDa
Observed band size: 53 kDa

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling p53 with ab131442 at 1/50 dilution. Right panel was preincubated with blocking peptide.

Immunofluorescent analysis of methanol-fixed HeLa cells staining p53 with ab131442 at 1/100 dilution.