Anti-mtTFA antibody (ab155240)
Key features and details
- Rabbit polyclonal to mtTFA
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-mtTFA antibody
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-246 of Human mtTFA (Q00059)
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Positive control
- WB: HEK-293T, A431, HeLa and HepG2 cell lysate; IHC-P: Human colon and esophagus cancer tissue; IF: HeLa whole cells; IP: HEK-293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-mtTFA antibody (ab155240) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 29 kDa12% SDS PAGE
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Immunohistochemical analysis of human colon cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling mtTFA protein at mitochondria (green) with ab155240 at 1/1000 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/10000 dilution. Blue: Hoechst 33342 staining.
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IP Sample:
1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, 40 µg;
2: Control with 2 μg of preimmune rabbit IgG;
3: Immunoprecipitation of mtTFA protein by 2 μg of ab155240.
The immunoprecipitated mtTFA protein was detected by ab155240 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
15% SDS-PAGE
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Immunohistochemical analysis of human esophagus cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.