Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-mtTFA antibody (ab155240)

Anti-mtTFA antibody (ab155240)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to mtTFA
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Product image
Anti-Oct6 antibody [EPR24057-94] (ab259952)
Product image
PE Anti-Mesothelin antibody [EPR19025-42] (ab252136)
Product image
Anti-CYP3A43 antibody (ab230048)
Product image
Recombinant Human SDHA protein (His tag) (ab226453)

Overview

  • Product name

    Anti-mtTFA antibody
    See all mtTFA primary antibodies
  • Description

    Rabbit polyclonal to mtTFA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-246 of Human mtTFA (Q00059)

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 cell lysate; IHC-P: Human colon and esophagus cancer tissue; IF: HeLa whole cells; IP: HEK-293T cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Images

  • Western blot - Anti-mtTFA antibody (ab155240)
    Western blot - Anti-mtTFA antibody (ab155240)
    All lanes : Anti-mtTFA antibody (ab155240) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 29 kDa



    12% SDS PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)

    Immunohistochemical analysis of human colon cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab155240)
    Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab155240)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling mtTFA protein at mitochondria (green) with ab155240 at 1/1000 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/10000 dilution. Blue: Hoechst 33342 staining.

  • Immunoprecipitation - Anti-mtTFA antibody (ab155240)
    Immunoprecipitation - Anti-mtTFA antibody (ab155240)

    IP Sample:

    1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, 40 µg;

    2: Control with 2 μg of preimmune rabbit IgG;

    3: Immunoprecipitation of mtTFA protein by 2 μg of ab155240.

    The immunoprecipitated mtTFA protein was detected by ab155240 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

    15% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)

    Immunohistochemical analysis of human esophagus cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-mtTFA antibody (ab155240)

  •  
  • Product image

    Anti-mtTFA antibody [EPR12286] (ab171951)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-mtTFA antibody [EPR12285] (ab176558)

    Applications: ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-mtTFA antibody (ab138351)

    Applications: WB

  •  
  • Product image

    HRP Anti-mtTFA antibody [EPR12285] (ab209022)

    Applications: WB

  •  
  • Product image

    Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-mtTFA antibody - Mitochondrial Marker (ab47517)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-mtTFA antibody [EPR12285] - BSA and Azide free (ab240958)

    Applications: ICC/IF, IHC-P, IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-MUC16 antibody [OC125] (ab693)

  •  
  • Product image

    Anti-Chd7 antibody (ab176807)

  •  
  • Product image

    Anti-ASF1A antibody (ab24171)

  •  
  • Product image

    Anti-TTC39B antibody (ab72982)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.