Anti-mtTFA antibody (ab138351)
Key features and details
- Rabbit polyclonal to mtTFA
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-mtTFA antibody
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Mouse mtTFA aa 50-150 conjugated to keyhole limpet haemocyanin.
Database link: P40630 -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; A431; Jurkat; MCF7; HepG2; Caco2; NIH3T3; PC12.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications Chinese hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7019 Human
- Entrez Gene: 21780 Mouse
- Entrez Gene: 83474 Rat
- Omim: 600438 Human
- SwissProt: Q00059 Human
- SwissProt: P40630 Mouse
- SwissProt: Q91ZW1 Rat
- Unigene: 594250 Human
see all -
Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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Western blot - Anti-mtTFA antibody (ab138351)All lanes : Anti-mtTFA antibody (ab138351) at 1 µg/ml (Milk blocking 1%)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab138351 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (4)
ab138351 has been referenced in 4 publications.
- Yambire KF et al. Mitochondrial biogenesis is transcriptionally repressed in lysosomal lipid storage diseases. Elife 8:N/A (2019). PubMed: 30775969
- Tomar D et al. Blockade of MCU-Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation. Cell Rep 26:3709-3725.e7 (2019). PubMed: 30917323
- Basheer WA et al. Stress response protein GJA1-20k promotes mitochondrial biogenesis, metabolic quiescence, and cardioprotection against ischemia/reperfusion injury. JCI Insight 3:N/A (2018). PubMed: 30333316
- Chung N et al. The effects of exercise and cold exposure on mitochondrial biogenesis in skeletal muscle and white adipose tissue. J Exerc Nutrition Biochem 21:39-47 (2017). PubMed: 28715885
Images
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Western blot - Anti-mtTFA antibody (ab138351)All lanes : Anti-mtTFA antibody (ab138351) at 1 µg/ml (Milk blocking 1%)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab138351 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
