Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ZIP Kinase antibody [EPR1635] (ab79422)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1635] to ZIP Kinase
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-ZIP Kinase antibody [EPR1635]
See all ZIP Kinase primary antibodies
Description

Rabbit monoclonal [EPR1635] to ZIP Kinase
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human ZIP Kinase aa 1-100 (N terminal).
Database link: O43293
Positive control
- WB: HEK293T, HeLa and A431 cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1635
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other

Western blot - Anti-ZIP Kinase antibody [EPR1635] (ab79422)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: DAPK3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab79422 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab79422 was shown to specifically react with DAPK3 in wild-type HAP1 cells along with other cross-reactive bands. No bands were observed when DAPK3 knockout samples were examined. Wild-type and DAPK3 knockout samples were subjected to SDS-PAGE. Ab79422 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ZIP Kinase antibody [EPR1635] (ab79422)

All lanes : Anti-ZIP Kinase antibody [EPR1635] (ab79422) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAPK3 knockout HEK293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab79422 observed at 53 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab79422 was shown to react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and DAPK3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab79422 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ZIP Kinase antibody [EPR1635] (ab79422)

All lanes : Anti-ZIP Kinase antibody [EPR1635] (ab79422) at 1/5000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa
Anti-ZIP Kinase antibody [EPR1635] (ab79422)

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