Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1636Y] to ZIP Kinase - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free
    See all ZIP Kinase primary antibodies

  • Description

    Rabbit monoclonal [EPR1636Y] to ZIP Kinase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes ZIP Kinase.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, HeLa and HEK-293T cell lysate.

  • General notes

    Ab247317 is the carrier-free version of ab51602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247317 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)
    Western blot - Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)

    This data was developed using ab51602, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: ZIP Kinase knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: A431 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab51602 was shown to specifically react with ZIP Kinase in wild-type HAP1 cells. No band was observed when ZIP Kinase knockout samples were examined. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Ab51602 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)
    Western blot - Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)
    All lanes : Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : DAPK3 knockout HEK293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab51602).

    Lanes 1- 2: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab51602 was shown to react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and DAPK3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)
    Western blot - Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)
    Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    This data was developed using ab51602, the same antibody clone in a different buffer formulation.

     

  • Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)
    Anti-ZIP Kinase antibody [EPR1636Y] - BSA and Azide free (ab247317)

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