Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Urokinase antibody [EPR6273] (ab133563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6273] to Urokinase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Urokinase antibody [EPR6273]
    See all Urokinase primary antibodies

  • Description

    Rabbit monoclonal [EPR6273] to Urokinase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Urokinase aa 50-150. The exact sequence is proprietary.
    Database link: P00749

  • Positive control

    • WB: PC-3, LnCaP and MCF7 cell lysates. IHC-P: Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Urokinase antibody [EPR6273] (ab133563)
    Western blot - Anti-Urokinase antibody [EPR6273] (ab133563)
    All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/100 dilution (unpurified)

    Lane 1 : LnCap cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urokinase antibody [EPR6273] (ab133563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Urokinase antibody [EPR6273] (ab133563)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Urokinase with purified ab133563 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-Urokinase antibody [EPR6273] (ab133563)
    Western blot - Anti-Urokinase antibody [EPR6273] (ab133563)
    All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/1300 dilution (purified)

    Lane 1 : LnCaP cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Urokinase antibody [EPR6273] (ab133563)
    Western blot - Anti-Urokinase antibody [EPR6273] (ab133563)
    All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/1000 dilution (unpurified)

    Lane 1 : PC-3 cell lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 49 kDa

  • OI-RD Scanning - Anti-Urokinase antibody [EPR6273] (ab133563)
    OI-RD Scanning - Anti-Urokinase antibody [EPR6273] (ab133563)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Urokinase antibody [EPR6273] (ab133563)
    Anti-Urokinase antibody [EPR6273] (ab133563)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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