Anti-Urokinase antibody (ab109055)
Key features and details
- Rabbit polyclonal to Urokinase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Urokinase antibody
See all Urokinase primary antibodies -
Description
Rabbit polyclonal to Urokinase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human prostate; Human Thyroid; Human Liver.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RabbitHorseMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Target
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- SwissProt: Q8MIL0 Rabbit
- Unigene: 77274 Human
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Alternative names
- ATF antibody
- ATF uPA antibody
- BDPLT5 antibody
see all
Images
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Western blot - Anti-Urokinase antibody (ab109055)All lanes : Anti-Urokinase antibody (ab109055) at 1 µg/ml
Lane 1 : Human prostate tissue lysate (cirrhosis) - total protein (ab30307)
Lane 2 : Human thyroid normal tissue lysate - total protein (ab29636)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 148 kDa, 34 kDa, 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References (0)
ab109055 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Urokinase antibody (ab109055)All lanes : Anti-Urokinase antibody (ab109055) at 1 µg/ml
Lane 1 : Human prostate tissue lysate (cirrhosis) - total protein (ab30307)
Lane 2 : Human thyroid normal tissue lysate - total protein (ab29636)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 148 kDa, 34 kDa, 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
