Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)
![Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)](https://www.abcam.com/ps/products/223/ab223542/Images/ab223542-3-ab205198272079IF1.jpg "Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17088-68] to AACT - BSA and Azide free
- Suitable for: IP, ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-AACT antibody [EPR17088-68] - BSA and Azide free
See all AACT primary antibodies -
Description
Rabbit monoclonal [EPR17088-68] to AACT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma (untreated and PNGase F treated) and blood; Human fetal spleen lysate. IHC-P: Human tonsil tissue. ICC/IF: Raji cells. IP: Human plasma lysate.
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General notes
Ab223542 is the carrier-free version of ab205198. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab223542 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17088-68 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) cells labeling AACT with ab205198 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on Raji cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab205198 at 1/50 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205198).
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![Immunoprecipitation - Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)](https://www.abcam.com/ps/products/223/ab223542/Images/ab223542-2-ab205198272078205198IP1.jpg)
Immunoprecipitation - Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)AACT was immunoprecipitated from 1 mg of Human plasma lysate with ab205198 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab205198 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1: Human plasma lysate, 10 μg (Input).
Lane 2: ab205198 IP in Human plasma lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205198 in Human plasma lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205198).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)](https://www.abcam.com/ps/products/223/ab223542/Images/ab223542-282796-ab205198IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)This IHC data was generated using the same anti-AACT antibody clone [EPR17088-68] in a different buffer formulation (cat# ab205198).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling AACT with ab205198 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab205198) at 1/500 dilution. Cytoplasmic staining on human tonsil tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)](https://www.abcam.com/ps/products/223/ab223542/Images/ab223542-4-benefits-of-recombinant-antibodies.png)
Anti-AACT antibody [EPR17088-68] - BSA and Azide free (ab223542)
