Anti-AACT antibody (ab88527)
Key features and details
- Mouse polyclonal to AACT
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AACT antibody
See all AACT primary antibodies -
Description
Mouse polyclonal to AACT -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length protein corresponding to Human AACT.
Database link: NP_001076.2 -
Positive control
- WB: HeLa cell lysate and AACT transfected 293T cell lysate IF: HeLa cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AACT antibody (ab88527)Anti-AACT antibody (ab88527) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 48 kDa
Observed band size: 48 kDa
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Western blot - Anti-AACT antibody (ab88527)All lanes : Anti-AACT antibody (ab88527) at 1 µg/ml
Lane 1 : AACT transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 48 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-AACT antibody (ab88527)ab88527 at 10 µg/ml staining AACT in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
