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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5351] to Rb (phospho T826)
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Rb (phospho T826) antibody [EPR5351]
    See all Rb primary antibodies
  • Description

    Rabbit monoclonal [EPR5351] to Rb (phospho T826)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Rb phosphorylated at Threonine 826.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide corresponding to a region surrounding Threonine 826 of Human Rb.

  • Positive control

    • Jurkat cell lysate treated with FBS.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 9.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5351
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Images

  • Western blot - Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)
    Western blot - Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)
    All lanes : Anti-Rb (phospho T826) antibody [EPR5351] (ab133446) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate treated with FBS.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)
    OI-RD Scanning - Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)
    Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Rb (phospho T826) antibody [EPR5351] (ab133446)

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Applications: Dot, ICC/IF, IHC-P, WB

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    Anti-Rb (phospho S780) antibody [EPR182(N)] (ab173289)

    Applications: Dot, IHC-P, WB

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    Anti-Rb antibody [EPR17512] - BSA and Azide free (ab218526)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-Rb (phospho T821) antibody [E231] (ab32015)

    Applications: IP, WB

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    Applications: WB

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