Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2153AY] to Rb (phospho T356) - BSA and Azide free
  • Suitable for: IHC-P, Dot blot, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EPR2153AY] to Rb (phospho T356) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only detects Rb phosphorylated on Threonine 356.

  • Tested applications

    Suitable for: IHC-P, Dot blot, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Jurkat whole cell lysate.

  • General notes

    ab247461 is the carrier-free version of ab76298 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247461 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298) at 1/4000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) 2 day serum starved whole cell lysate.
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate.
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate. Membrane incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 1 minute


    This data was developed using ab76298, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

    This data was developed using ab76298, the same antibody clone in a different buffer formulation.

    ab76298 showing positive staining in Endometrial carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Immunoprecipitation - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

    This data was developed using ab76298, the same antibody clone in a different buffer formulation.
    Purified ab76298 at 1/20 dilution (0.5µg) immunoprecipitating Rb in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab76298 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76298 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa
    Cleavage fragments were observed as described in literature. (PMID: 11420704)

  • Dot Blot - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Dot Blot - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

    This data was developed using ab76298, the same antibody clone in a different buffer formulation.

    Dot blot analysis of Rb (phospho T356) phospho peptide (Lane 1), Rb Non-phospho peptide (Lane 2), labelling Rb (phospho T356) with ab76298 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

    This data was developed using ab76298, the same antibody clone in a different buffer formulation.

    ab76298 showing positive staining in Lung adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

    This data was developed using ab76298, the same antibody clone in a different buffer formulation.

    ab76298 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    This data was developed using ab76298, the same antibody clone in a different buffer formulation.ab76298 showing positive staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    This data was developed using ab76298, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76298 at 1/100-1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Western blot - Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate treated with FBS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa



    This data was developed using ab76298, the same antibody clone in a different buffer formulation.

  • Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)
    Anti-Rb (phospho T356) antibody [EPR2153AY] - BSA and Azide free (ab247461)

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