Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Occludin antibody [EPR8208] (ab167161)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8208] to Occludin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Occludin antibody [EPR8208]
    See all Occludin primary antibodies

  • Description

    Rabbit monoclonal [EPR8208] to Occludin

  • Host species

    Rabbit

  • Specificity

    The antibody does not recognize human Occludin isoform 4 and isoform 5 based on the immunogen sequence.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Occludin aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16625

  • Positive control

    • WB: Human fetal kidney tissue lysate. HepG2, HUVEC, Rat-1, and bEnd.3 whole cell lysates. Mouse and rat spinal cord and small intestine tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8208

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Occludin antibody [EPR8208] (ab167161)
    Western blot - Anti-Occludin antibody [EPR8208] (ab167161)
    All lanes : Anti-Occludin antibody [EPR8208] (ab167161) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
    Lane 3 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : C57 mouse spinal cord lysate
    Lane 6 : Mouse small intestine lysate
    Lane 7 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
    Lane 8 : Rat spinal cord lysate
    Lane 9 : Rat small intestine lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent as what is described in PMID: 17332770, PMID: 24884662 and PMID: 15054114.
    Although some papers support the expression of Occludin in HUVEC (PMID:31057027, 29927318) and bEnd.3 (PMID: 31096970), ab167161 cannot detect band of interest in these cell lines.
    The expression of occludin in brain, spinal cord and intestine is consistent with what has been described in PMID: 31511021, PMID: 30742658 and PMID: 29927318.

    Blocking/Diluting buffer: 5% NFDM/TBST

    Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

  • Western blot - Anti-Occludin antibody [EPR8208] (ab167161)
    Western blot - Anti-Occludin antibody [EPR8208] (ab167161)
    All lanes : Anti-Occludin antibody [EPR8208] (ab167161) at 1/50000 dilution (unpurified)

    Lane 1 : Human fetal kidney cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa

  • Western blot - Anti-Occludin antibody [EPR8208] (ab167161)
    Western blot - Anti-Occludin antibody [EPR8208] (ab167161)
    Lane 1 : Anti-Occludin antibody [EPR8208] (ab167161) at 1/5000 dilution (unpurified)
    Lane 2 : Anti-Occludin antibody (ab31721) at 1/1000 dilution (unpurified)
    Lane 3 : Anti-Occludin antibody [EPR20992] (ab216327) at 1/5000 dilution (unpurified)

    All lanes : Recombinant Human Occludin protein (ab114189)

    Lysates/proteins at 0.025 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time:     5.5 seconds
  • Anti-Occludin antibody [EPR8208] (ab167161)
    Anti-Occludin antibody [EPR8208] (ab167161)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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