Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Claudin 1 antibody [EPR9306] (ab180158)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9306] to Claudin 1
Suitable for: WB
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Claudin 1 antibody [EPR9306]
See all Claudin 1 primary antibodies
Description

Rabbit monoclonal [EPR9306] to Claudin 1
Host species

Rabbit
Specificity

Claudin-1 expression is minimal in normal colon tissues and is increased in tumors (PMID: 17308096,PMID: 15965503).
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Claudin 1 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: O95832
Positive control
- WB: A431, Human skin and fetal liver lysates, Mouse kidney and liver tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9306
Isotype

IgG
Research areas

Western blot - Anti-Claudin 1 antibody [EPR9306] (ab180158)

All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/10000 dilution

Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : CLDN1 knockout A431 whole cell lysate
Lane 3 : A549 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 23 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab180158 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180158 was shown to specifically react with in wild-type A431 WT cells as signal was lost in A431 CLDN1 knockout cells. Wild-type and A431 CLDN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab180158 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Claudin 1 antibody [EPR9306] (ab180158)

Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/2000 dilution + Human fetal liver lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 23 kDa
Western blot - Anti-Claudin 1 antibody [EPR9306] (ab180158)

All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/10000 dilution

Lane 1 : A431 cell lysate
Lane 2 : Human skin lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa
Western blot - Anti-Claudin 1 antibody [EPR9306] (ab180158)

All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse colon tissue lysate
Lane 4 : Mouse small intestine tissue lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Exposure time: 60 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Claudin-1 expression is minimal in normal colon tissues and is increased in tumors (PMID: 17308096, PMID: 15965503).
Anti-Claudin 1 antibody [EPR9306] (ab180158)

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