Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Claudin 1 antibody [CL3698] (ab242370)

Key features and details

Mouse monoclonal [CL3698] to Claudin 1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-Claudin 1 antibody [CL3698]
See all Claudin 1 primary antibodies
Description

Mouse monoclonal [CL3698] to Claudin 1
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Claudin 1 aa 150 to the C-terminus.
Database link: O95832

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human liver, small intestine, placenta, prostate, prostate cancer, breast cancer, liver cancer and colorectal cancer tissue. WB: RT4 cell lysate. ICC/IF: A431 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL3698
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [CL3698] (ab242370)

PFA-fixed, Triton X-100 permeabilized A431 cells stained for CLCN1 (Green) using ab242370 at 4 μg/ml in ICC/IF.

Microtublues are stained red, nuclei are stained blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human liver tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Western blot - Anti-Claudin 1 antibody [CL3698] (ab242370)

All lanes : Anti-Claudin 1 antibody [CL3698] (ab242370) at 1 µg/ml

Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

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