Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Claudin 1 antibody [CL3698] (ab242370)

Key features and details

  • Mouse monoclonal [CL3698] to Claudin 1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Claudin 1 antibody [CL3698]
    See all Claudin 1 primary antibodies

  • Description

    Mouse monoclonal [CL3698] to Claudin 1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Claudin 1 aa 150 to the C-terminus.
    Database link: O95832

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human liver, small intestine, placenta, prostate, prostate cancer, breast cancer, liver cancer and colorectal cancer tissue. WB: RT4 cell lysate. ICC/IF: A431 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    CL3698

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [CL3698] (ab242370)

    PFA-fixed, Triton X-100 permeabilized A431 cells stained for CLCN1 (Green) using ab242370 at 4 μg/ml in ICC/IF.

    Microtublues are stained red, nuclei are stained blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human liver tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Western blot - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Western blot - Anti-Claudin 1 antibody [CL3698] (ab242370)
    All lanes : Anti-Claudin 1 antibody [CL3698] (ab242370) at 1 µg/ml

    Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [CL3698] (ab242370)

    Formalin-fixed, paraffin-embedded human small intestine tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

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