Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Occludin antibody [CL1555] (ab242202)

Key features and details

Mouse monoclonal [CL1555] to Occludin
Suitable for: ICC, IHC-P, WB
Reacts with: Human
Isotype: IgG2a

Product name

Anti-Occludin antibody [CL1555]
See all Occludin primary antibodies
Description

Mouse monoclonal [CL1555] to Occludin
Host species

Mouse

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Occludin aa 250-450.
Database link: Q16625

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptide sequence TSPVDDFRQPRYSSG as determined by overlapping synthetic peptides.
Positive control
- WB: Caco-2 cell lysate. ICC: Caco-2 cells. IHC-P: Human stomach, kidney, colon, fallopian tube, liver, and prostate cancer tissue..
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL1555
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-Occludin antibody [CL1555] (ab242202)

PFA-fixed, Triton X-100 permeabilized CACO-2 cells stained for OCLN (Green) using ab242202 at 4 μg/ml in ICC/IF.

Microtublues are stained red, nuclei are stained blue.
Western blot - Anti-Occludin antibody [CL1555] (ab242202)

All lanes : Anti-Occludin antibody [CL1555] (ab242202) at 1 µg/ml

Lane 1 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Formalin-fixed, paraffin-embedded human colon tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Formalin-fixed, paraffin-embedded human liver tissue stained for Occludin with ab242202 at a 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [CL1555] (ab242202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeeltal muscle tissue labelling Occludin with ab242202 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

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