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Signal Transduction Cytoskeleton / ECM Cell Adhesion Tight Junctions

Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8208] to Occludin - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Occludin antibody [EPR8208] - BSA and Azide free
    See all Occludin primary antibodies
  • Description

    Rabbit monoclonal [EPR8208] to Occludin - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney tissue lysate. HepG2, HUVEC, Rat-1, and bEnd.3 whole cell lysates. Mouse and rat spinal cord and small intestine tissue lysates.
  • General notes

    Ab240150 is the carrier-free version of ab167161. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240150 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8208
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions

Images

  • Western blot - Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)
    Western blot - Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)
    All lanes : Anti-Occludin antibody [EPR8208] (ab167161) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
    Lane 3 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : C57 mouse spinal cord lysate
    Lane 6 : Mouse small intestine lysate
    Lane 8 : Rat spinal cord lysate
    Lane 9 : Rat small intestine lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent as what is described in PMID: 17332770, PMID: 24884662 and PMID: 15054114.
    Although some papers support the expression of Occludin in HUVEC (PMID:31057027, 29927318) and bEnd.3 (PMID: 31096970), ab167161 cannot detect band of interest in these cell lines. 
    The expression of occludin in brain, spinal cord and intestine is consistent with what has been described in PMID: 31511021, PMID: 30742658 and PMID: 29927318.

    Blocking/Diluting buffer: 5% NFDM/TBST

    Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab167161).

  • Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)
    Anti-Occludin antibody [EPR8208] - BSA and Azide free (ab240150)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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