Anti-CRB3 antibody [14F9] (ab180835)
![Anti-CRB3 antibody [14F9] (ab180835)](https://www.abcam.com/ps/products/180/ab180835/Images/ab180835-209561-anti-crb3-antibody-14f9-immunofluorescence.jpg "Anti-CRB3 antibody [14F9] (ab180835)")
Key features and details
- Rat monoclonal [14F9] to CRB3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2c
Overview
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Product name
Anti-CRB3 antibody [14F9] -
Description
Rat monoclonal [14F9] to CRB3 -
Host species
Rat -
Specificity
The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Rat CRB3.
Database link: 301112 -
Positive control
- Mouse kidney and colon tissue lysates; HEK293T cells overexpressed with CRB3. Mouse intestine tissue. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
PFHM culture media -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Lyophilized protein G purified in PBS pH7.4 -
Clonality
Monoclonal -
Clone number
14F9 -
Isotype
IgG2c -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)
Immunofluorescence analysis of HeLa cell labeling CRB3 with ab1808350 at a 1/100 dilution. DAPI was used to stain the nuclei.
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![Western blot - Anti-CRB3 antibody [14F9] (ab180835)](https://www.abcam.com/ps/products/180/ab180835/Images/ab180835-209559-anti-crb3-antibody-14f9-western-blot.png)
Western blot - Anti-CRB3 antibody [14F9] (ab180835)All lanes : Anti-CRB3 antibody [14F9] (ab180835) at 1/1000 dilution
Lane 1 : HEK293T cells overexpressed with control vector
Lane 2 : HEK293T cells overexpressed with CRB3
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse colon tissue lysate
Predicted band size: 13 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)](https://www.abcam.com/ps/products/180/ab180835/Images/ab180835-209560-anti-crb3-antibody-14f9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (ab180835)Immunofluorescence analysis of Mouse intestine labeling CRB3 with ab1808350 at a 1/100 dilution. E-cadherin staining was used to highlight the lateral membrane in mouse intestine.