Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AKAP3 antibody [EPR11261] (ab170856)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11261] to AKAP3
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-AKAP3 antibody [EPR11261]
See all AKAP3 primary antibodies
Description

Rabbit monoclonal [EPR11261] to AKAP3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human AKAP3. The exact sequence is proprietary.
Database link: O75969
Positive control
- Y79, HuT-78, HeLa, 293T, NIH 3T3, NCI-H460 and Jurkat whole cell lysate (ab7899); HeLa cells; Permeabilized Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11261
Isotype

IgG
Research areas

Western blot - Anti-AKAP3 antibody [EPR11261] (ab170856)

All lanes : Anti-AKAP3 antibody [EPR11261] (ab170856) at 1/1000 dilution

Lane 1 : Y79 cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lane 5 : NIH 3T3 cell lysate
Lane 6 : NCI-H460 cell lysate
Lane 7 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 95 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AKAP3 antibody [EPR11261] (ab170856)

Immunofluorescent analysis of HeLa cells labeling AKAP3 with ab170856 at 1/250 dilution (red). DAPI nuclear staining (blue).
Flow Cytometry - Anti-AKAP3 antibody [EPR11261] (ab170856)

Flow cytometric analysis of permeabilized Jurkat cells labeling AKAP3 with ab170856 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Anti-AKAP3 antibody [EPR11261] (ab170856)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com