Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC2 antibody [EPR6145] (ab134119)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6145] to MUC2
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-MUC2 antibody [EPR6145]
    See all MUC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6145] to MUC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human MUC2.

  • Positive control

    • WB: Caco2, SKBR3, HT1376, SH SY5Y and SK OV3 cell lysates and human small intestine and colon lysates. IHC-P: Human colon tissue.

  • General notes

    Mouse and rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-MUC2 antibody [EPR6145] (ab134119)
    Western blot - Anti-MUC2 antibody [EPR6145] (ab134119)
    All lanes : Anti-MUC2 antibody [EPR6145] (ab134119) at 1/2000 dilution ((unpurified))

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HT-1376 (Human urinary bladder carcinoma epithelial cell) whole cell lysate
    Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 5 : Human small intestine tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 540 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The 110kda band is the C-terminal cleaved fragment, formed by an autocatalytic mechanism triggered by low pH. (PMID: 12582180).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-MUC2 antibody [EPR6145] (ab134119)
    ELISA - Anti-MUC2 antibody [EPR6145] (ab134119)

    Indirect ELISA using ab134119 at varying antibody concentrations (1000-0 ng/mL) and MUC2 antigen at 1000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with unpurified ab134119 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MUC2 antibody [EPR6145] (ab134119)
    Western blot - Anti-MUC2 antibody [EPR6145] (ab134119)
    Anti-MUC2 antibody [EPR6145] (ab134119) at 1/10000 dilution (Purified) + Human colon lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 540 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    MUC2 could be cleaved into three fragments with the molecular weight 250, 130 and 110 KD as is described in PMID: 12582180.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)

    Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using unpurified ab134119 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)

    Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab134119 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MUC2 antibody [EPR6145] (ab134119)
    OI-RD Scanning - Anti-MUC2 antibody [EPR6145] (ab134119)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using unpurified ab134119 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab134119 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (ab134119)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using unpurified ab134119 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-MUC2 antibody [EPR6145] (ab134119)
    Anti-MUC2 antibody [EPR6145] (ab134119)

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