Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6145] to MUC2 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free
    See all MUC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6145] to MUC2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Caco2, SKBR3, HT1376, SH SY5Y and SK OV3 cell lysates and human small intestine and colon lysates. IHC-P: Human colon tissue.

  • General notes

    ab177278 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119)
  • ELISA - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    ELISA - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    indirect ELISA using ab134119 at varying antibody concentrations (1000-0 ng/ml) and MUC2 antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

    This IHC data was generated using the same anti-MUC2 antibody clone, EPR6145, in a different buffer formulation (cat# ab134119).

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with ab134119 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

    This IHC data was generated using the same anti-MUC2 antibody clone, EPR6145, in a different buffer formulation (cat# ab134119).

    Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134119 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

    Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134119 showing -ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab134119 showing -ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab134119 showing -ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab134119 showing -ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    OI-RD Scanning - Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134119).

  • Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)
    Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

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